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UBE2S (NM_014501) Human Recombinant Protein

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SKU
TP720259
Recombinant protein of human ubiquitin-conjugating enzyme E2S (UBE2S)
Size:
10 ug 500 ug 50 ug 1 mg
  $160.00
7 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
Met1-Leu222
Tag N-GST
Predicted MW 50.1 kDa
Concentration lot specific
Purity >95% as determined by SDS-PAGE and Coomassie blue staining
Buffer Supplied as a 0.2 um filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
Endotoxin < 0.1 EU per µg protein as determined by LAL test
Storage Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Stability Stable for at least 3 months from date of receipt under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_055316
Locus ID 27338
UniProt ID Q16763
Cytogenetics 19q13.42
Synonyms E2-EPF; E2EPF; EPF5
Summary This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
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Documents
Resources
Other "UBE2S" proteins (4)
SKU Description Size Price
PH324851 UBE2S MS Standard C13 and N15-labeled recombinant protein (NP_055316) 10 ug
$3,360.00
LC402342 Lyophilized UBE2S HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402342 Transient overexpression lysate of ubiquitin-conjugating enzyme E2S (UBE2S) (as WB positive control) 100 ug
$436.00
TP324851 Recombinant protein of human ubiquitin-conjugating enzyme E2S (UBE2S), 20 µg 20 ug
$867.00

file-alt Citations

Blocking mutant IDH1 phosphorylation triggers APC/C CDH1-dependent ubiquitination in mitotic cells
Swain, Jain
Biochim Biophys Acta Mol Cell Res (2025) 1872 (7), 120002 DOI: 10.1016/j.bbamcr.2025.120002
Cited by 1
AI Summary: In this study, the OriGene product Ube2S E2 (SKU TP720259) was used in in vitro ubiquitination assays to investigate how blocking phosphorylation of mutant IDH1 facilitates its APC/C CDH1-dependent ubiquitination in mitotic cells.
Snippet: ween to remove endogenous Ube2S. The beads were incubated with 5 μM IDH1R132H-T77A-S94A, 1 μM Ubiquitin E1 (SRP6147, Sigma), 1 μM Ube2S E2 (SKU TP720259, Origene), 100 μM His-Ubiquitin, 10 mM DTT, 3 mM ATP, and 22.5 mM creatine phosphate in 25 mM Tris·HCl (pH 7.5), 50 mM NaCl, and 10 mM MgCl2 at 30 °C for 2 h. Samples were resolved on 8 % SDS/PAGE, and polyubiquitinated protein was detected using His-HRP. …
Showing 1-1 of 1 papers.
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