CPSF73 (CPSF3) (NM_016207) Human Recombinant Protein

SKU TP701246

Purified recombinant protein of Human cleavage and polyadenylation specific factor 3, 73kDa (CPSF3), mutant（D75K, H76A）, with C-terminal MYC/DDK tag, expressed in HEK293 cells, 20ug

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from TrueORF clone, [RC205834], encoding the full-length of CPSF3(D75K, H76A)
• Tag: Myc-DDK
• Predicted MW: 80.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate Bradford method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_057291
• Locus ID: 51692
• UniProt ID: Q9UKF6
• Cytogenetics: 2p25.1
• RefSeq Size: 2401
• RefSeq ORF: 2052
• Synonyms: CPSF-73; CPSF73
• Summary: This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]