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Hace1 (NM_172473) Mouse Recombinant Protein

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SKU
TP525877
Purified recombinant protein of Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR225877 protein sequence
Red=Cloning site Green=Tags(s)

MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH
IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV
NGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQR
DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHL
SQQSESQYLKILTGLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNK
TPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSTDITSILLKQKGQDQEAPSLSAFEPPGPGSYESLP
PGPGDSKPEVLAGEQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF
VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRD
SIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN
SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS
DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP
SLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSR
VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 102.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_766061
Locus ID 209462
UniProt ID Q3U0D9
Cytogenetics 10 B2
RefSeq Size 3785
RefSeq ORF 2727
Synonyms 1700042J16Rik; A730034A22Rik; BC025474
Summary E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins, Nervous system: Neurodegenerative diseases
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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