Plb1 (NM_001081407) Mouse Recombinant Protein

CAT#: TP517244

Purified recombinant protein of Mouse phospholipase B1 (Plb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR217244 representing NM_001081407
  Red=Cloning site Green=Tags(s)
  
  MELYPGVSPVGLLLLLLLGQGPSQIHGSSGENTLAWQSQQVFWTLKNFPFPCKPKKLELSVLSESVHSLR
  PSDIKLVAAIGNPEIPLAPGSGTINMEKPQSIKNQPQDVCMGIMTVLSDIIRHFSPSVLMPTCSPGKGTA
  VHTTAEDLWIQAKELVRRLKDNPQLDFEKDWKLITVFFSNTSQCHLCPSAQQKSHLMRHMEMLWGVLDYL
  HHEVPRAFVNLVDLSEVLAMDLQHQETGFSPAPEVCKCTETTTLSKAVMQWSYQEAWEDLLASSKFNKHE
  TFAVVFQPFFDEIEPPLKRSSPQDPTTLALRIWNSMMEPVGQKDGLLNTAERKTMKCPSEESPYLFTYKN
  SNYQARRLKPITKLQMKEGSEFTCPDKNPSNSIPTTVHSLRPADIKIIGALGDSLTAGNGAGASPWNILD
  VLTEYRGLSWSVGGDETIKTVTTLPNILREFNPSLKGFSVGTGKESTSRASFNQAVAGAKSDGLAGQARK
  LVDLMKADKTINFQEDWKIITVFIGGNDLCASCSNSTRFSPQNFIDNIKNALDILHAEVPRAFVNMAMVM
  EITPLRELFNEPTVSCPRNILSRLCPCVLGLGDNSEELSSLVQRNRDYQKKTEELINSGRYDTRDNFTVV
  VQPLFENVSMPRTPEGVPDKSFFAPDCFHFNAKTHARSAIALWKNMLEPVGHKTRHNNFEIKAPIVCPNQ
  ASPFLSTTKNSNLGNGTWMVCEERAPSASPPTSVHTLRPADIQVVAALGDSLTAGNGISSQEGNLTDVST
  QYRGLSYSAGGDKTLENVTTLPNILRKFNGNLTGYSVGTGDSSSANAFLNQAVPGAKAENLTSQVRTLVQ
  KMKSDNRVNFNRDWKVITVMIGASDLCDFCTDSNHYSAANFFDHLQNALDILHKEVPRALVNLVDFINPS
  IIREVFLKNPDKCPVNQSSVLCNCVLTPRKDSYELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQP
  FLLNTKLPVLENGKPDTSFFAPDCIHLNQKFHTQLARALWANMLEPLGKKTDTLDPKGHISLACPTKDQP
  FLRTFRNSNYKYPTKPAIENWGSDFLCTEKSPSSQVPTSVHELRPADIKVVAAMGDFLTTATGARPSGYK
  RLATPWRGLSWSIGGDGKLETHTTLPNILKKFNPSITGFSTGTLDNKAGLNVAEEGARAQDMPAQAKTLV
  KKMKSTPTINLQEDWKLITLLIGNNDLCLYCENPEDNSTKEYVKYIQQALDILYEELPRVFINVVEVMEL
  AGLHHVQGGKCAMPLAVQKNCSCLRHSQNLTAMQELKKLNWNLQSGISELSYWHRYMEREDFAVTVQPFF
  RNTFIPLNEREGLDLTFFSEDCFYFSDRGHAEMAIALWNNMLEPVGWKTSSNNFIYNRTKLKCPSPERPF
  LYTLRNSQLLPDKAEEPSNALYWAVPVAAIGGLAVGILGVMLWRTVKPVQQEEEEEDTLPNTSVTQDAVS
  EKRLKAGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 165 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001074876
• Locus ID: 665270
• UniProt ID: Q3TTY0
• Cytogenetics: 5 B1
• Refseq Size: 4853
• Refseq ORF: 4434
• Synonyms: 4632413E21Rik; 4930433E17Rik; 4930539A06Rik; BC033606
• Summary: Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).[UniProtKB/Swiss-Prot Function]