Amot (NM_153319) Mouse Recombinant Protein

CAT#: TP515464

Purified recombinant protein of Mouse angiomotin (Amot), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR215464 representing NM_153319
  Red=Cloning site Green=Tags(s)
  
  MRSSDDQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNSSPFSTGSGNQGPQNDVLSSQDHHQQ
  QLVAHPARQEPQGQEIQSENGVMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQ
  KMRTEGRPSVQRLTPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYK
  NATSSSEFYKAQGPPPSQHSLKGMEHRGPPPEYPFKGVPSQSVVCKSQEPGHFYSEHRLNQPGRTEGQLM
  RYQHPPEYGAARATQDISSLSLSARNSQPHSPTSSLTAGASSLPLLQSPPSTRLPPGQHLVSNQGDHSAH
  LSRHQQHLLSSQSHQGDHYRHAQASLTSAQQQPGEAYSAMPRAQQSASYQPMPADPFAMVSRAQQMVEIL
  SDENRNLRQELDGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDL
  RDRLETANKQLAEKEYEGSEDTRKTISQLFAKHKENQREKEKLEAELATARSTNEDQRRHIEIRDQALSN
  AQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNSQPTN
  ASEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDT
  ALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSSMRPAKSLMSI
  SNAGSGLLAHSSTLTGAPIMEEKRDDKSWKGSLGVLLGGDYRVEPVPSTPSPVPPSTPLLSAHSKTGSRD
  CSTQTERGPESTKTAAVTPISAPMAGPVAAAAPAAAINATAATNTATAATNTTIMVAAAPVAVAAVAAPA
  AAAATPSPANAAALAAAAAPATSVSAATSVSAANSISPAAPVAPAAVVPPAAPVSPAAAVQIPAAASLTP
  ATVSPTAATATAAVAAATTAAITAAAAAATTAIQVAPATSAPVPSPASIPAPATAQASAPTPTQASTPAP
  TEPPSPVPTPTPALVQTEGPANPGASSGPRRLSTPNLMCNPDKPDAPAFHSSTLERKTPIQILGQEPDAE
  MVEYLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 120.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_695231
• Locus ID: 27494
• UniProt ID: Q8VHG2
• Cytogenetics: X F2
• Refseq Size: 6974
• Refseq ORF: 3378
• Synonyms: CAG-2; D0Kist1; Sii6
• Summary: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function]