Aqr (NM_009702) Mouse Recombinant Protein

CAT#: TP512006

Purified recombinant protein of Mouse aquarius (Aqr), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR212006 representing NM_009702
  Red=Cloning site Green=Tags(s)
  
  MAAPAQPKKIVAPTVSQINAEFVTQLACKYWAPHIKKKSPFDIKVIEEIYEKEIVKSRFAIRKIMLLEFS
  QYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWETFKKKPDHFPFFFKCILKAALAETDGEFSL
  HEQTLLLLFLDHCFNSLEVDLIRSQVQQLISLPMWMGLQPARLELELKKTPKLRKFWNLIKKNDEKMDPE
  AREQAYQERRFLSRLIQKFISVLKSIPLSEPVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLL
  VHCYLSSLVHREEDGHLFSQLLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQRAAFAHFPELYD
  FALSNVAEVDARDSLVKFFGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQIQQ
  LNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLRNFNLFRLESTYEIRQDIEDSVS
  RMKPWQSEYGGVVFGGWARMAQPIVAFTVVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHD
  VCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEDGPEPRPNLRGESRTFRVFLD
  PNQYQQDMTNTIQNGAEDVYDTFNVIMRRKPKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSA
  HYSKMPNQIATLDFNDTFLSIEHLKASFPGHNVKVTVSDPALQIPPFRITFPVRSGKGKKRKDADGEEDD
  TEEAKTLIVEPHVIPNRGPYPYNQPKRNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNI
  YHNFPEQRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHGEEELETEKDFSRYGRVNYVLARRIELLE
  EVKRLQKSLGVPGDASYTCETAGYFFLYQVMSRWEEYMSRVKNSGTACPDAAPDAAQVATFFPFHEYFAN
  APQPIFKGRSYEEDMEIAEGCFRHIKKIFTQLEEFRASELLRSGLDRSKYLLVKEAKIIAMTCTHAALKR
  HDLVKLGFKYDNILMEEAAQILEIETFIPLLLQNPQDGFSRLKRWIMIGDHHQLPPVIKNMAFQKYSNME
  QSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQ
  GVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNK
  VTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFARVSLFQNCFELTPAFSQLTAR
  PLHLHIIPTEPFPTSRKNGERPPHEVQVIKNMPQMANFVYNMYMHLIQTTHHYHQTFLQLPPAMVEEGEE
  GQSQETEMEAEEETVSAQGNLTPSPADASLSQETPAAQPDCSSQTEDTSAPCDIATAAEPVSAAAEAATP
  QDAESVPTETE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 170.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_033832
• Locus ID: 11834
• UniProt ID: Q8CFQ3
• Cytogenetics: 2 57.57 cM
• Refseq Size: 4897
• Refseq ORF: 4443
• Synonyms: AW495846; mKIAA0560
• Summary: Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro).[UniProtKB/Swiss-Prot Function]