Kdm3a (NM_001038695) Mouse Recombinant Protein

CAT#: TP511928

Purified recombinant protein of Mouse lysine (K)-specific demethylase 3A (Kdm3a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211928 representing NM_001038695
  Red=Cloning site Green=Tags(s)
  
  MVLTLGESWPVLVGKRFLSLSAAEGNEGGQDNWDLERVAEWPWLSGTIRAVSHTDVTKKDLKVCVEFDGE
  SWRKRRWIDVYSLQRKAFLVEHNLVLAERKSPEVPEQVIQWPAIMYKSLLDKAGLGAITSVRFLGDQQSV
  FVSKDLLKPIQDVNSLRLSLTDNQTVSKEFQALIVKHLDESHLLQGDKNLVGSEVKIYSLDPSTQWFSAT
  VVHGNPSSKTLQVNCEEIPALKIVDPALIHVEVVHDNFVTCGNSTRTGAVKRKSSENNGSSVSKQAKSCS
  EASPSMCPVQSVPTTVFKEILLGCTAATPSSKDPRQQNTPQAANSPPNIGAKLPQGCHKQNLPEELSSCL
  NTKPEVPRTKPDVCKEGLLSSKSSQVGAGDLKILSEPKGSCIQPKTNTDQESRLESAPQPVTGLPKECLP
  AKTSSKAELDIATTPELQKHLEHAASTSDDLSDKPEVKAGVTSLNSCAEKKVEPSHLGSQSQNLKETSVK
  VDNESCCTRSSNKTQTPPARKSVLTDPDKVRKLQQSGEAFVQDDSCVNIVAQLPKCRECRLDSLRKDKDQ
  QKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDSEAIGLWLPLTKNVVGTDLDTAKYILANIGDHFC
  QMVISEKEAMSTIEPHRQVAWKRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAA
  YKTFSWIRCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPALKEDL
  KQTSLSGEKPTLGTMVQQSSPVLEPVAVCGEAASKPASSVKPTCPTSTSPLNWLADLTSGNVNKENKEKQ
  LTMPILKNEIKCLPPLPPLNKPSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTAKTENGLKNTPKILDDI
  FASLVQNKTSSDSSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNKSNWNVFRECWKQGQPVMVSG
  VHHKLNTELWKPESFRKEFGEQEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNDKEKEPMVLKLKDW
  PPGEDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTN
  LHLDVSDAANVMVYVGIPKGQCEQEEEVLRTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREF
  LKKVSEEQGQDNPADHDPIHDQSWYLDRSLRKRLYQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCI
  KVAEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSLGKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 148.3 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001033784
• Locus ID: 104263
• UniProt ID: Q6PCM1
• Cytogenetics: 6 C1
• Refseq Size: 4829
• Refseq ORF: 3969
• Synonyms: 1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
• Summary: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function]