Sh3bp4 (NM_133816) Mouse Recombinant Protein

CAT#: TP511323

Purified recombinant protein of Mouse SH3-domain binding protein 4 (Sh3bp4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211323 representing NM_133816
  Red=Cloning site Green=Tags(s)
  
  MAAQRIRAANASGLPRCKSEGTLIDLSEGFSETSFNDVKVPSPSALLVDNPTPFGNAKEVIAIKDYCPNN
  FTTLKFSKGDHLYVLDTSGGEWWYAHNTTEMGYIPSSYVQPLNYRNSTLSDSGMIDNLPDSPEEVAKELD
  LLGGGWTDDQKESGRPYSNNPFWNGVRTNPFLNGNAQPSTDELNPKSTVDLLLFDTGTSSFTESSSATTN
  STGNIFDELPATNGLQVEQPVKRDNPFFRSKRSYSLSELSVLQAKSDAPPTSSFFTGLKSPVPEQFQSRE
  DFRTAWLNHRKLARSCHDLDLLGQSPGWGQTQAVETNIVCKLDSSGGSVQLPDTNISIHVPEGHVAPGET
  QQISMKALLDPPLDLNSDRSTSVSPVVEVKLSNLEVSTFIILEMKVSAEVKGDIFSKSTVVLQCLRSDSK
  EGPYVPIPLAYSYGDTIQVQLDNLEPCMYLAIVAQGPNILYPSTVWDFINKRVTVGLYGPKHIHPSFKTV
  VTIFGHDCAPKTLLVSEVTRQAPSPAPVALQLWGKHQFILSRPQDLRVCMFSNMTNYEVKANEQARVVRG
  FQMKLGKVSRLIFSVISQNPNELSDFTLRVQVKDDQDTILTQFCVQTPQPPPKSAIKPSGQRRFLKKNEV
  GKIILSPFVVTTKYPTFQDRPVSSLKFGKLLKTVVRQNKSHYLLEYKKGDVVALLSEERIRLKGQLWTKE
  WYIGYYQGKVGLVHTKNVLVVGKARPSLFSGPELSTSVLLEQILRPCKFLTYIYASVRTLLMENISSWRA
  FADALGYGNLPLTFFCRAELDSEPERVASVLEKLKEDCNNPDNKDRKSFQKELVMALLKMDCQGLVVRLI
  QDFVLLTTAVEVAQRWRELAEKLAKVSKQQMDAYESPHRDRNGVVDSEAMWKPAYDFLLTWSHQIGDSYR
  DVIQELHIGLDKMKNPITRRWKHLTGTLILVNSLDILRAAAFSPADHDDFVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 108 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_598577
• Locus ID: 98402
• UniProt ID: Q921I6
• Cytogenetics: 1 D
• Refseq Size: 3673
• Refseq ORF: 2886
• Synonyms: AI594717; AW227605; BOG25
• Summary: May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).[UniProtKB/Swiss-Prot Function]