Rapgef3 (NM_144850) Mouse Recombinant Protein

CAT#: TP511040

Purified recombinant protein of Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211040 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEDSLDFRVSLEQATTEHVHKAGKLLHRHLLATY
  PTLIRDRKYHLRLYRHCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFY
  RFPGPEPEPTGTQDVEEELVEAMALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNS
  VKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRA
  ATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGT
  PEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPSTQLFTALLHHFHVEPADPAGGSEQEHSTYIC
  NKRQQILRLVGRWVALYSPMLHSDPVATSFLQKLSDLVSRDARLSNLLREQYPERRRHHRLENGCGNVSP
  QTKARNAPVWLPNQEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQ
  VLVKVNSAGDVVGLQPDARGVATSLGLNERLFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQ
  LTDHDWNLFNRIHQVQEHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGSRAQLLRKFIKLAAHLKE
  QKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPL
  LLKDVTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQGSRISTCSE
  QSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 99.2 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_659099
• Locus ID: 223864
• UniProt ID: Q8VCC8
• Cytogenetics: 15 F1
• Refseq Size: 3801
• Refseq ORF: 2631
• Synonyms: 2310016P22Rik; 9330170P05Rik; Epac; Epac1
• Summary: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin (By similarity).[UniProtKB/Swiss-Prot Function]