Plekhg6 (NM_198604) Mouse Recombinant Protein

CAT#: TP510656

Purified recombinant protein of Mouse pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210656 representing NM_198604
  Red=Cloning site Green=Tags(s)
  
  MQAFGPPNEGPLQGLVASRIETYGGRHQTSAHSTAGNLFPRGGPGVDPSRRRLQHYVPFAKGSGQTRGMS
  PLVLREPDPEKRHGSYFGVGPPHSPKLKEVTRAHELEIRLHTFSMFGMPRLPPEDRRHWEIGEGGDSALT
  MEKSWKELVLEHKEMNRQLCHQQEALWELLTTELIYLRKLKIMTDLLAAGLLNLQRVGLLTEVSAETLFG
  NVPNLIRAHRRFWEEVLQPILEDTRTSGQPLDPVSLQNGFLTFGQRFQPYVQYCLRVKQTMAYAREQQDT
  NPLFHTFVQWCEKHKRSGRQTLGDLLIKPHQRITKYPLLLQAVRKRSPEPRAQEALNAMIEAVESFLRHI
  NGQVRQGEEQESLMAVAQRIGPYEVLEPSSEEVEKNLRPFSTLDLMTPVLGVAPEYTRQLLLEGPVRVKE
  GREGKMDVYLFLFSDVLLVTKPQRKADRAKVIRPPLMLEKLVCRPLRDPNSFLLIHLTEFQCVSSALTVH
  CPSSTERARWLEKTQHAQTTLQKLKGEQYIQQKRELLALYRNQGTESPSTRPSTPSPSPEDSQSSAEGRT
  LEFAIIPRLVVTEDTDEDTPSMPDDASDSGYGTLIPSSPKDSHSPLNRLRSKALRRDPRLTFSTLELRDV
  PLRPQPPDPQAPQRRSAPELPEGILRGGSLPRRAPPIWSEEEDETLASGNVVVETLHRAQRRSPLPHSPT
  HTDSAGESPWESSDEDEGLLSPELRPRSLREDMLREIREELANQRIDGASEPEPGNGKPRRLTLAQLQRM
  RVPHIIQLDTPLSTSEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 89.1 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_941006
• Locus ID: 213522
• UniProt ID: Q8R0J1
• Cytogenetics: 6 F3
• Refseq Size: 3019
• Refseq ORF: 2361
• Synonyms: BC026778; Gm155
• Summary: Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis (By similarity).[UniProtKB/Swiss-Prot Function]