Sema4f (NM_011350) Mouse Recombinant Protein

CAT#: TP510594

Purified recombinant protein of Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210594 representing NM_011350
  Red=Cloning site Green=Tags(s)
  
  MLARAERPRPGPRPPPVSLFPPPSSLLLLLLAMLSAPVCGRVPRSVPRTSLPISEADSYLTRFAAPHTYN
  YSALLVDPASHTLYVGARDSIFALTLPFSGEKPRRIDWMVPETHRQNCRKKGKKEDECHNFIQILAIANA
  SHLLTCGTFAFDPKCGVIDVSSFQQVERLESGRGKCPFEPAQRSAAVMAGGVLYTATVKNFLGTEPIISR
  AVGRAEDWIRTETLSSWLNAPAFVAAMVLSPAEWGDEDGDDEIFFFFTETSRVLDSYERIKVPRVARVCA
  GDLGGRKTLQQRWTTFLKADLLCPGPEHGRASGVLQDMTELRPQPGAGTPLFYGIFSSQWEGAAISAVCA
  FRPQDIRAVLNGPFRELKHDCNRGLPVMDNEVPQPRPGECITNNMKFQQFGSSLSLPDRVLTFIRDHPLM
  DRPVFPADGRPLLVTTDTAYLRVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLALFPET
  QPVESMKLYHDWLLVGSHTEVTQVNTSNCGRLQSCSECILAQDPVCAWSFRLDACVAHAGEHRGMVQDIE
  SADVSSLCPKEPGEHPVVFEVPVATVGHVVLPCSPSSAWASCVWHQPSGVTSLTPRRDGLEVVVTPGAMG
  AYACECQEGGAARVVAAYSLVWGSQRGPANRAHTVVGAGLVGFFLGVLAASLTLLLIGRRQQRRRQRELL
  ARDKVGLDLGAPPSGTTSYSQDPPSPSPEDERLPLALGKRGSGFGGFPPPFLLDSCPSPAHIRLTGAPLA
  TCDETSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 84.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_035480
• Locus ID: 20355
• UniProt ID: Q9Z123
• Cytogenetics: 6 35.94 cM
• Refseq Size: 4067
• Refseq ORF: 2331
• Synonyms: Sema W
• Summary: This gene encodes a member of semaphorin family of membrane-bound and secreted proteins that are involved in guiding axonal growth. The encoded protein is a transmembrane protein localized to the glutamatergic synapses via its association with a synapse-associated scaffolding protein. In oligodendrocyte precursor cells, the encoded protein contributes to the outward migration and differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]