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Glb1 (NM_009752) Mouse Recombinant Protein

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SKU
TP509735
Purified recombinant protein of Mouse galactosidase, beta 1 (Glb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR209735 protein sequence
Red=Cloning site Green=Tags(s)

MLRVPLCTPLPLLALLQLLGAAHGIYNVTQRTFKLDYSRDRFLKDGQPFRYISGSIHYFRIPRFYWEDRL
LKMKMAGLNAIQMYVPWNFHEPQPGQYEFSGDRDVEHFIQLAHELGLLVILRPGPYICAEWDMGGLPAWL
LEKQSIVLRSSDPDYLVAVDKWLAVLLPKMKPLLYQNGGPIITVQVENEYGSYFACDYDYLRFLVHRFRY
HLGNDVILFTTDGASEKMLKCGTLQDLYATVDFGTGNNITQAFLVQRKFEPKGPLINSEFYTGWLDHWGK
PHSTVKTKTLATSLYNLLARGANVNLYMFIGGTNFAYWNGANTPYEPQPTSYDYDAPLSEAGDLTKKYFA
LREVIQMFKEVPEGPIPPSTPKFAYGKVALRKFKTVAEALGILCPNGPVKSLYPLTFTQVKQYFGYVLYR
TTLPQDCSNPKPIFSSPFNGVRDRAYVSVDGVPQGILDRNLMTALNIQGKAGATLDILVENMGRVNYGRF
INDFKGLISNMTINSTVLTNWTVFPLDTEAMVRNHLWGREASDGGHLDGRSTSNSSDLILPTFYVGNFSI
PSGIPDLPQDTFIQFPGWSKGQVWINGFNLGRYWPTMGPQKTLFVPRNILTTSAPNNITVLELEFAPCSE
GTPELCTVEFVDTPVIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 73 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_033882
Locus ID 12091
UniProt ID P23780
Cytogenetics 9 64.4 cM
RefSeq Size 2396
RefSeq ORF 1941
Synonyms AW125515; Bg; Bge; Bgl; Bgl-; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik
Summary This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a lysosomal enzyme that catalyzes the hydrolysis of terminal beta-D-galactose residues in various substrates like lactose, ganglioside GM1 and other glycoproteins. Mutations in the human gene are associated with GM1-gangliosidosis and Morquio B syndrome. Disruption of the mouse gene mirrors the symptoms of human gangliosidosis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2013
Protein Categories Intracellular Proteins
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Documents
Resources

file-alt Citations

A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
Crippa, Alberti, Passerini et al
Molecular Therapy - Methods & Clinical Development (2024) 32 (3), 101313 DOI: 10.1016/j.omtm.2024.101313
Cited by 4
AI Summary: OriGene’s recombinant murine β-GAL (product TP509735) was used to generate a calibration curve for enzymatic activity assays in the study, enabling accurate measurement of β-GAL activity in gene therapy experiments for mucopolysaccharidosis type IVB.
Snippet: CM untransduced and transduced (LV-Glb1 WT, LV-Glb1 C2C12, and LV-GLB1) HEK293T cells, a calibration curve was built upon testing the enzymatic activity of serial dilutions of a 10 μL solution containing known concentration of recombinant human (rh) (Biotechne, 6464-GH) or murine (rm) (Origene, TP509735) β-GAL. …
Preclinical development of hematopoietic stem cell-gene therapy for Mucopolysaccharidosis type IVB using a GLB1 transgene with enhanced therapeutic potential
Crippa, Alberti, Passerini et al
Thesis (2024)
Snippet: HEK293T cells, a calibration curve was built upon testing the enzymatic activity of serial dilutions of a 10 μL solution containing known concentration of recombinant human (rh) (Biotechne, 6464-GH) or murine (rm) (Origene, TP509735) β-GAL. A logarithmic trendline best fitted results of rm β-GAL activity, while linear correlation best fitted rh β-GAL up to 350 nmol/mg/h. …
Showing 1-2 of 2 papers.
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