Gtf2e1 (NM_028812) Mouse Recombinant Protein

SKU TP507009

Purified recombinant protein of Mouse general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR207009 protein sequence; Red=Cloning site Green=Tags(s); ; MADPDVLTEVPAALKRLAKYVIRGFYGIEHVLALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDK; FIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERNSTNRASFKCPVCCSTFTDLE; ANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTE; IPALKQSKDRAATAAGAAGLAGGHHREAWTNKGPSYEDLYTQNVVINMDDQDDVHRPSLEGKAAKERPIW; LRESTVQGAYSSEEMKEGGIDVDTFQEREEARAGPDDNEEVMRALLIHEKKTSSVTAGSVGAAAPVTAAN; GSDSESETSESDDDSPPRPAAAAPPHHHRDEDEEDEEFEEVADDPIVMVAGCPFSYSEVSQRPELVAQMT; PEEKEAYIAMGQRMFEDLFE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 49.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_083088
• Locus ID: 74197
• UniProt ID: Q9D0D5
• Cytogenetics: 16 B3
• RefSeq Size: 3058
• RefSeq ORF: 1320
• Synonyms: 56kDa; 2610024P03Rik; AI303800; FE; TFIIE-A
• Summary: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]