Myosin Phosphatase (PPP1R12A) (NM_002480) Human Recombinant Protein
SKU
TP323540
Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 115.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002471 |
| Locus ID | 4659 |
| UniProt ID | O14974 |
| Cytogenetics | 12q21.2-q21.31 |
| RefSeq Size | 4613 |
| RefSeq ORF | 3132 |
| Synonyms | GUBS; M130; MBS; MYPT1 |
| Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2009 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Myosin Phosphatase" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH323540 | PPP1R12A MS Standard C13 and N15-labeled recombinant protein (NP_002471) | 10 ug |
$3,360.00
|
|
| PH327493 | PPP1R12A MS Standard C13 and N15-labeled recombinant protein (NP_001137357) | 10 ug |
$3,360.00
|
|
| LC400883 | Lyophilized PPP1R12A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC428392 | Lyophilized PPP1R12A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400883 | Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| LY428392 | Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| TP327493 | Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 12A (PPP1R12A), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
Using Quantitative Seroproteomics to Identify Antibody Biomarkers in Pancreatic Cancer
Jhaveri, Kim, Thompson et al
Cancer Immunol Res (2016) 4 (3), 225-33 DOI: 10.1158/2326-6066.CIR-15-0200-T
Jhaveri, Kim, Thompson et al
Cancer Immunol Res (2016) 4 (3), 225-33 DOI: 10.1158/2326-6066.CIR-15-0200-T
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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