DPH5 (NM_015958) Human Recombinant Protein

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SKU
TP320529
Recombinant protein of human DPH5 homolog (S. cerevisiae) (DPH5), transcript variant 2, 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC220529 representing NM_015958
Red=Cloning site Green=Tags(s)

MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILK
DADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTW
RPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEE
PAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQ
SINGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 31.5 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_057042
Locus ID 51611
UniProt ID Q9H2P9
Cytogenetics 1p21.2
RefSeq Size 1457
RefSeq ORF 855
Synonyms AD-018; CGI-30; HSPC143; NPD015
Summary This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
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Other "DPH5" proteins (5)
SKU Description Size Price
PH320529 DPH5 MS Standard C13 and N15-labeled recombinant protein (NP_057042) 10 ug
$3,360.00
LC414310 Lyophilized DPH5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421406 Lyophilized DPH5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY414310 Transient overexpression lysate of DPH5 homolog (S. cerevisiae) (DPH5), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY421406 Transient overexpression lysate of DPH5 homolog (S. cerevisiae) (DPH5), transcript variant 3 (as WB positive control) 100 ug
$436.00

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