JAK2 (NM_004972) Human Recombinant Protein
SKU
TP320503M
Recombinant protein of human Janus kinase 2 (a protein tyrosine kinase) (JAK2), 100 µg
$2,950.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 130.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004963 |
| Locus ID | 3717 |
| UniProt ID | O60674 |
| Cytogenetics | 9p24.1 |
| RefSeq Size | 5097 |
| RefSeq ORF | 3396 |
| Synonyms | JTK10 |
| Summary | This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2020 |
| Protein Categories | Cancer: Haematopoietic and Lymphoid, Cardiovascular diseases, Cytokines, Enzyme: Kinases, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
iper nigrum L. and its main component piperine ameliorate hyperuricemia and hyperuricemia-related renal inflammation through suppressing the JAK2-STAT3-XDH/SOCS3/TNF-α signalling pathway
Li, Xin, Song et al
Food & Medicine Homology (2026)
Li, Xin, Song et al
Food & Medicine Homology (2026)
AI Summary: OriGene products, including the pcDNA-JAK2 plasmid (TP320503) and anti-DDK Tag antibody (AE005), were used in this study to investigate the molecular mechanisms by which Piper nigrum L. and piperine modulate the JAK2-STAT3 signaling pathway in hyperuricemia and related renal inflammation.
TonEBP inhibits ciliogenesis by controlling aurora kinase A and regulating centriolar satellite integrity
Chinbold, Kwon, Park
Cell Commun Signal (2024) 22 (1), 348 DOI: 10.1186/s12964-024-01721-8
Chinbold, Kwon, Park
Cell Commun Signal (2024) 22 (1), 348 DOI: 10.1186/s12964-024-01721-8
Cited by 3
AI Summary: In this study, the OriGene chicken anti-mCherry antibody (TA150127) was used to detect mCherry-tagged TonEBP, enabling the researchers to analyze the localization and effects of TonEBP overexpression on ciliogenesis.
Snippet: ), mouse Alexa Fluor 647 anti-gamma (γ) tubulin (ab191114, Abcam), mouse anti-acetylated tubulin (T7451, Sigma-Aldrich), rabbit anti-ARL13b (17711-1-AP, Proteintech), chicken anti-mCherry (TA150127, OriGene), rabbit anti-aurora kinase A (4718, Cell Signaling), rabbit anti-phosphorylated aurora kinase A (2914, Cell Signaling), rabbit anti-Rab11 (71-5300, Invitrogen), rabbit anti-CP110 (ab243696, A
Cellular spermine targets JAK signaling to restrain cytokine-mediated autoimmunity
Xu, Zhang, Wang et al
Immunity (2024)
Xu, Zhang, Wang et al
Immunity (2024)
Cited by 69
A CK2-dependent mechanism for activation of the JAK-STAT signaling pathway
Zheng, Qin, Frank et al
Blood (2011) 118 (1), 156-66 DOI: 10.1182/blood-2010-01-266320
Zheng, Qin, Frank et al
Blood (2011) 118 (1), 156-66 DOI: 10.1182/blood-2010-01-266320
Cited by 134
AI Summary: OriGene contributed recombinant human full-length JAK2 protein to this study, enabling the authors to investigate the interaction and phosphorylation between JAK2 and CK2 in the activation of the JAK-STAT signaling pathway.
Snippet: and/or CK2β, as well as the pharmacologic inhibitors 4,5,6,7-tetrabromobenzotriazole (TBB) and emodin to knock down CK2 expression or ... Recombinant human full-length JAK2 was purchased from OriGene Technologies, and recombinant human CK2 (α and β) was from New
Showing 1-4 of 4 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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