ULK1 (NM_003565) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 112.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_003556 |
| Locus ID | 8408 |
| UniProt ID | O75385 |
| Cytogenetics | 12q24.33 |
| RefSeq Size | 5228 |
| RefSeq ORF | 3150 |
| Synonyms | ATG1; ATG1A; hATG1; UNC51; Unc51.1 |
| Summary | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).UniProtKB/Swiss-Prot Function |
| Protein Categories | Enzyme: Kinases, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | mTOR signaling pathway, Regulation of autophagy |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "ULK1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH315643 | ULK1 MS Standard C13 and N15-labeled recombinant protein (NP_003556) | 10 ug |
$3,360.00
|
|
| LC418585 | Lyophilized ULK1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY418585 | Transient overexpression lysate of unc-51-like kinase 1 (C. elegans) (ULK1) (as WB positive control) | 100 ug |
$665.00
|
|
Citations
JAK2/ULK1 axis promotes cervical cancer progression by autophagy induction and SRPK1 phosphorylation
Duan, Wang, Xiong et al
Oncogene (2024)
Duan, Wang, Xiong et al
Oncogene (2024)
Cited by 4
Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer
Xu, Chen, Yan et al
Oncol Rep (2024) 52 (4) DOI: 10.3892/or.2024.8784
Xu, Chen, Yan et al
Oncol Rep (2024) 52 (4) DOI: 10.3892/or.2024.8784
Cited by 1
AI Summary: OriGene products, including the serum blocking solution (ZLI-9056), antibody dilution buffer (ZLI-9030), biotinylated secondary antibody (PV-9000-1), and streptavidin-enzyme conjugate (PV-9000-2), were used to prepare and visualize tissue sections in immunohistochemistry experiments, enabling the study of ZNF180 and METTL14 protein expression in colorectal cancer.
Snippet: After rinsing under cold tap water for 10 min, the sections were incubated for 10 min at room temperature with 10% serum blocking solution (cat. no. ZLI-9056;OriGene Technologies, Inc.). Subsequently, the sections were incubated with the following primary antibodies: ZNF180 (cat. no. NBP1-92613;Novus Biologicals, Inc.) and METTL14 (cat. no. 26158-1-AP;Proteintech Group, Inc.) in a 1:400 dilution i...
Urolithin A Hijacks ERK1/2-ULK1 Cascade to Improve CD8+ T Cell Fitness for Antitumor Immunity
Ma, Wu, Wu et al
Adv Sci (Weinh) (2024)
Ma, Wu, Wu et al
Adv Sci (Weinh) (2024)
Cited by 35
Structure-Based Virtual Screening towards the Discovery of Novel ULK1 Inhibitors with Anti-HCC Activities
Gao, Zhou, Zhang et al
Molecules (2022) 27 (9) DOI: 10.3390/molecules27092627
Gao, Zhou, Zhang et al
Molecules (2022) 27 (9) DOI: 10.3390/molecules27092627
Cited by 15
AI Summary: OriGene products, specifically the ULK1 expression plasmid (RC217674) and ULK1 shRNA plasmid (TG311115), were used in this study to manipulate ULK1 expression in HCC cells, enabling the investigation of ZZY-19’s mechanism of action as a ULK1 inhibitor.
MAPK1/3 kinase-dependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis
Deng, Zhang, Huang et al
Autophagy (2020)
Deng, Zhang, Huang et al
Autophagy (2020)
Cited by 237
AI Summary: OriGene's Human recombinant ULK1 protein (catalog number TP315643) was used in this study to investigate the mechanisms of ULK1 phosphorylation and degradation, contributing to the understanding of how mitophagy defects promote breast cancer bone metastasis.
Snippet: The function of mitophagy in cancer is controversial. ULK1 is critical for induction of macroautophagy/autophagy and has a more specific role in mitophagy in response to hypoxia. Here, we show that...;Human recombinant ULK1 (TP315643, OriGene) was incubated with or without (negative controls) human recombinant activated MAPK3/ERK1 (Merck Millipore, 14-439)
Showing 1-5 of 5 papers.
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