Dynamitin (DCTN2) (NM_006400) Human Recombinant Protein

CAT#: TP314771

Recombinant protein of human dynactin 2 (p50) (DCTN2), 20 µg

Product Images

Tau-dynactin co-immunoprecipitation indicates that hyperphosphorylation of tau increases its affinity toward dynactin-2. Dynactin-2 (OriGene TP314771), pre-incubated with Dynabeads-G and an anti-dynactin-2 antibody, were incubated with native or hyperphosphorylated tau (via reaction with GSK-3) and the pulldown samples were analyzed with Western blot through a Tau antibody. Figure cited from Front Neurosci, PMID: 27857681

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  Coomassie blue staining of purified DCTN2 protein (Cat# TP314771). The protein was produced from HEK293T cells transfected with DCTN2 cDNA clone (Cat# RC214771) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC214771 representing NM_006400
  Red=Cloning site Green=Tags(s)
  
  MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAFAQELEELTSTSVEHIIVNPNAAYDKFKDKRVGTK
  GLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTP
  VLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLV
  TYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAV
  LDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQ
  FGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 44.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Bioactivity: Co-immunoprecipitation (PMID: 27857681)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_006391
• Locus ID: 10540
• UniProt ID: Q13561, V9HW58
• Cytogenetics: 12q13.3
• Refseq Size: 1757
• Refseq ORF: 1218
• Synonyms: DCTN50; DYNAMITIN; HEL-S-77; RBP50
• Summary: This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
• Protein Pathways: Huntington's disease