EIF4G1 (NM_004953) Human Recombinant Protein
SKU
TP312877
Purified recombinant protein of Homo sapiens eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 154.6 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | In vitro translation assay (PMID: 25779044) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004944 |
| Locus ID | 1981 |
| UniProt ID | Q04637 |
| Cytogenetics | 3q27.1 |
| RefSeq Size | 5045 |
| RefSeq ORF | 4209 |
| Synonyms | EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18 |
| Summary | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. provided by RefSeq, Aug 2010 |
| Protein Categories | Growth Factors, Intracellular Proteins, Nervous system: Neurodegenerative diseases |
| Protein Pathways | Viral myocarditis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "EIF4G1" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH312877 | EIF4G1 MS Standard C13 and N15-labeled recombinant protein (NP_004944) | 10 ug |
$3,360.00
|
|
| PH319841 | EIF4G1 MS Standard C13 and N15-labeled recombinant protein (NP_937884) | 10 ug |
$3,360.00
|
|
| LC404962 | Lyophilized EIF4G1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC417630 | Lyophilized EIF4G1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY404962 | Transient overexpression lysate of eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| LY417630 | Transient overexpression lysate of eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5 (as WB positive control) | 100 ug |
$665.00
|
|
| TP319841 | Purified recombinant protein of Homo sapiens eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
DAP5 associates with eIF2β and eIF4AI to promote Internal Ribosome Entry Site driven translation
Liberman, Gandin, Svitkin et al
Nucleic Acids Res. (2015)
Liberman, Gandin, Svitkin et al
Nucleic Acids Res. (2015)
Cited by 97
AI Summary: OriGene’s purified recombinant eIF4GI (transcript variant 5; Catalog # TP312877) was used as a control protein to investigate the specific interactions and functions of DAP5 in promoting IRES-driven translation in this study.
Snippet: Proteins were kept in 25 mM Tris-HCl [pH 8.0], 150 mM NaCl, 1 mM PMSF, 3 mM DTT and 5% glycerol. Purified recombinant eIF4GI, transcript variant 5 was purchased from OriGene (OriGene Technologies;Catalog # TP312877). ... Cite SaveSaving
CELF1 is an EIF4E binding protein that promotes translation of epithelial-mesenchymal transition effector mRNAs
Chaudhury, Pal, Kongchan et al
bioRxiv (2019)
Chaudhury, Pal, Kongchan et al
bioRxiv (2019)
Showing 1-2 of 2 papers.
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