PHLPP2 (NM_015020) Human Recombinant Protein
SKU
TP312846M
Recombinant protein of human PH domain and leucine rich repeat protein phosphatase-like (PHLPPL), 100 µg
$2,508.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 146.6 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_055835 |
| Locus ID | 23035 |
| UniProt ID | Q6ZVD8 |
| Cytogenetics | 16q22.2 |
| RefSeq Size | 8317 |
| RefSeq ORF | 3969 |
| Synonyms | PHLPPL; PPM3B |
| Summary | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606).UniProtKB/Swiss-Prot Function |
| Protein Categories | Cytokines, Intracellular Proteins |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Citations
Phosphatase PHLPP2 regulates the cellular response to metabolic stress through AMPK
Yan, Krecke, Bapat et al
Cell Death Dis (2021) 12 (10), 904 DOI: 10.1038/s41419-021-04196-4
Yan, Krecke, Bapat et al
Cell Death Dis (2021) 12 (10), 904 DOI: 10.1038/s41419-021-04196-4
Cited by 19
AI Summary: OriGene's purified recombinant PHLPP2 protein was used in in-vitro phosphatase assays to demonstrate that PHLPP2 directly dephosphorylates and inactivates AMPK, supporting the study’s findings on the regulatory role of PHLPP2 in cellular metabolic stress responses.
Snippet: xpanded and gene knockout was confirmed with TIDE [47] and Western blot analyses. In-vitro phosphatase assay FLAG-tagged PHLPP2, immunoprecipitated from cell lysates, or purified recombinant PHLPP2 (Origene Technologies, Rockville, MD), was incubated with 25 ng of active recombinant AMPKα1β1γ2 (ThermoFisher) for 15 min at 30 °C in assay buffer containing 0.05 M Tris-HCL PH7.4, 1 mM
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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