MACROH2A1 (NM_004893) Human Recombinant Protein

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SKU
TP310576
Recombinant protein of human H2A histone family, member Y (H2AFY), transcript variant 2, 20 µg
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC210576 protein sequence
Red=Cloning site Green=Tags(s)

MSSRGGKKKSTKTSRSAKAGVIFPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARD
NKKGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKGKLEAIITPPPAKKAKSPS
QKKPVSKKAGGKKGARKSKKKQGEVSKAASADSTTEGTPADGFTVLSTKSLFLGQKLNLIHSEISNLAGF
EVEAIINPTNADIDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKFVIHCNSPV
WGADKCEELLGKTVKNCLALADDKKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVY
FVLFDSESIGIYVQEMAKLDAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 39.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_004884
Locus ID 9555
UniProt ID O75367
Cytogenetics 5q31.1
RefSeq Size 1923
RefSeq ORF 1116
Synonyms H2A.y; H2A/y; H2AF12M; H2AFY; MACROH2A1.1; macroH2A1.2; mH2A1
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Oct 2015
Protein Categories Intracellular Proteins
Protein Pathways Systemic lupus erythematosus
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Other "MACROH2A1" proteins (6)
SKU Description Size Price
PH310576 H2AFY MS Standard C13 and N15-labeled recombinant protein (NP_004884) 10 ug
$3,360.00
LC417679 Lyophilized H2AFY HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421700 Lyophilized H2AFY HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417679 Transient overexpression lysate of H2A histone family, member Y (H2AFY), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY421700 Transient overexpression lysate of H2A histone family, member Y (H2AFY), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP761258 Purified recombinant protein of Human H2A histone family, member Y (H2AFY), transcript variant 4, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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