DDX1 (NM_004939) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 82.3 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Biolayer interferometry (BLI) assay (PMID: 25496916) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004930 |
| Locus ID | 1653 |
| UniProt ID | Q92499 |
| Cytogenetics | 2p24.3 |
| RefSeq Size | 2755 |
| RefSeq ORF | 2220 |
| Synonyms | DBP-RB; UKVH5d |
| Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. provided by RefSeq, Jul 2008 |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | Druggable Genome |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DDX1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308769 | DDX1 MS Standard C13 and N15-labeled recombinant protein (NP_004930) | 10 ug |
$3,360.00
|
|
| LC417641 | Lyophilized DDX1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY417641 | Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (DDX1) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Chemical profiling of DNA G-quadruplex-interacting proteins in live cells
Zhang, Spiegel, Martínez Cuesta et al
Nat Chem (2021) 13 (7), 626-633 DOI: 10.1038/s41557-021-00736-9
Zhang, Spiegel, Martínez Cuesta et al
Nat Chem (2021) 13 (7), 626-633 DOI: 10.1038/s41557-021-00736-9
Cited by 165
AI Summary: OriGene products, specifically RBM22 (TP760056) and Myc/DDK-tagged DDX1 (TP308769), were used as recombinant proteins in ELISA assays to study and validate the interactions between candidate proteins and DNA G-quadruplex structures in this research.
Snippet: H00029128-P01) and DDX24 (Abnova, catalogue no. H00057062-P01), HIS-tagged SMARCA4 (Abcam, catalogue no. ab82237), RBM22 (OriGene, TP760056) and Myc/DDK-tagged DDX1 (OriGene, TP308769) in ELISA buffer (100 mM KCl and 50 mM KH 2 PO 4
A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1
Lin, Sivakumaran, Jones et al
Retrovirology (2014) 11 (1), 121 DOI: 10.1186/PREACCEPT-7500780551442434
Lin, Sivakumaran, Jones et al
Retrovirology (2014) 11 (1), 121 DOI: 10.1186/PREACCEPT-7500780551442434
AI Summary: OriGene’s recombinant DDX1 and DDX3 proteins were used in this study to investigate and confirm the direct interaction between the HIV-1 Tat mutant Nullbasic and the cellular helicase DDX1, elucidating how Nullbasic disrupts HIV-1 Rev function.
Snippet: Recombinant proteins Recombinant DDX1 and DDX3 proteins were purchased from OriGene. The recombinant Nullbasic- FLAG-V5-6xHis was produced in E. coli strain BL21-AI (Invitrogen) transformed by pDEST42-Nullbasic-FLAG. A 200 ml culture was grown in Luria broth to log phase and induced with 0.2% arabinose and 1 mM IPTG
Showing 1-2 of 2 papers.
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