DARS2 (NM_018122) Human Recombinant Protein
SKU
TP307360
Recombinant protein of human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 73.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_060592 |
| Locus ID | 55157 |
| UniProt ID | Q6PI48 |
| Cytogenetics | 1q25.1 |
| RefSeq Size | 3187 |
| RefSeq ORF | 1935 |
| Synonyms | ASPRS; LBSL; MT-ASPRS; mtAspRS |
| Summary | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). provided by RefSeq, Nov 2009 |
| Protein Categories | Enzyme: Ligase, Intracellular Proteins, Nervous system: Neurodegenerative diseases |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DARS2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH307360 | DARS2 MS Standard C13 and N15-labeled recombinant protein (NP_060592) | 10 ug |
$3,360.00
|
|
| LC413308 | Lyophilized DARS2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY413308 | Transient overexpression lysate of aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Secreted mitochondrial aspartyl-tRNA synthetase (DARS2) regulates TNFα signaling
Johnson, Cornwell, Farkas et al
Physiol Rep (2025) 13 (21), e70627 DOI: 10.14814/phy2.70627
Johnson, Cornwell, Farkas et al
Physiol Rep (2025) 13 (21), e70627 DOI: 10.14814/phy2.70627
Cited by 1
AI Summary: OriGene contributed recombinant human DARS2 protein (catalog no. TP307360), which was used to investigate the effects of secreted DARS2 on macrophage activation in the study.
Snippet: n‐X100 (Sigma‐Aldrich, catalog no. 93443, in PBS)), and then eluted in 2× Laemmli (Bio‐Rad, catalog no. 1610737) via boiling. 2.9Administration of recombinant proteins Recombinant human DARS2 (OriGene Technologies, Inc., catalog no. TP307360) was packaged in Lipofectamine 3000 (Invitrogen, catalog no. L3000001) according to the manufacturer's instructions. These reactions were suspended in
Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses
Johnson, Farkas, El-Mergawy et al
Nat Commun (2024) 15 (1), 6172 DOI: 10.1038/s41467-024-50031-7
Johnson, Farkas, El-Mergawy et al
Nat Commun (2024) 15 (1), 6172 DOI: 10.1038/s41467-024-50031-7
Cited by 7
AI Summary: OriGene contributed recombinant human DARS2, AARS2, and DARS1 proteins to this study, which were used to investigate the molecular interactions and immune functions of mitochondrial aspartyl-tRNA synthetase in the context of bacterial pneumonia.
Snippet: LAG-tag 1:1000 dilution, (both from Cell Signaling), HA-Tag 1:2000 dilution (Novus), and Tim23 1:1000 dilution (Santa Cruz Biotechnology). The recombinant human proteins DARS2, AARS2, DARS1 were from Origene and E1, E2 were purchased from Enzo Life Sciences. Plasmids include Skp-1 (GenScript, NM_170679.3), Cullin1 (AddGene, 19896), and Rbx (AddGene, 20717). An aptamer-based protein array was purch
Showing 1-2 of 2 papers.
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