Pyruvate Dehydrogenase E2 (DLAT) (NM_001931) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 69.0 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_001922 |
| Locus ID | 1737 |
| UniProt ID | P10515 |
| Cytogenetics | 11q23.1 |
| RefSeq Size | 3321 |
| RefSeq ORF | 1941 |
| Synonyms | DLTA; E2; PBC; PDC-E2; PDCE2 |
| Summary | This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.provided by RefSeq, Oct 2009 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Pyruvate Dehydrogenase E2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH306640 | DLAT MS Standard C13 and N15-labeled recombinant protein (NP_001922) | 10 ug |
$3,360.00
|
|
| LC419633 | Lyophilized DLAT HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY419633 | Transient overexpression lysate of dihydrolipoamide S-acetyltransferase (DLAT), nuclear gene encoding mitochondrial protein (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Chemical Probes Reveal Sirt2's New Function as a Robust "Eraser" of Lysine Lipoylation
Xie, Chen, Wang et al
J. Am. Chem. Soc. (2019) 141 (46), 18428-18436 DOI: 10.1021/jacs.9b06913
Xie, Chen, Wang et al
J. Am. Chem. Soc. (2019) 141 (46), 18428-18436 DOI: 10.1021/jacs.9b06913
AI Summary: OriGene's human recombinant Pyruvate dehydrogenase E2 (DLAT) protein (NM_001931) was used in this study to investigate Sirt2's role in regulating lysine lipoylation and the activity of endogenous PDH.
Snippet: …Yao,6 Liang Zhang,2,4* Quan Hao,3* and Hongyan Sun1;Sirtuins, including Sirt1, Sirt2, Sirt3, Sirt5, and Sirt6, were recombinantly expressed and purified according to previous reports.(12b,c,17) Pyruvate dehydrogenase E2 (DLAT) (NM_001931) human recombinant protein was from ORIGENE. …
Showing 1-1 of 1 papers.
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