Pyruvate Dehydrogenase E2 (DLAT) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | WB: 200-1000 WB positive control: Lncap, hepg2 and A431 cell IHC: 25-100 Positive control: Human liver cancer Predicted cell location: Cytoplasm |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human DLAT |
| Buffer | pH7.4 PBS, 0.05% NaN3, 40% Glyceroln |
| Concentration | lot specific |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 69 kDa |
| Gene Name | dihydrolipoamide S-acetyltransferase |
| Database Link | |
| Background | This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood. |
| Synonyms | DLTA; PDC-E2; PDCE2 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism |
Reviews
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Citations
Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer
Becirovic, Zhang, Lindskog et al
Cell Death Discov (2024) 10 (1), 342 DOI: 10.1038/s41420-024-02111-2
Becirovic, Zhang, Lindskog et al
Cell Death Discov (2024) 10 (1), 342 DOI: 10.1038/s41420-024-02111-2
Cited by 10
AI Summary: OriGene's USP9x plasmid vector was used to overexpress USP9x in cell lines, enabling the authors to investigate its role in regulating the proline biosynthesis pathway in non-small cell lung cancer.
Snippet: purchased from Origene. At 80% confluency, the cells were transfected in 6-well plates with either pCMV empty vector or USP9x plasmid vector using the ViaFect transfection reagent (Promega, Madison, WI, USA) following the manufacturer’s protocol. The transfection mix contained 100 µL of Opti-MEM medium, 2 µg of the plasmid vector and 4 µL of ViaFect per 1 mL of cell culture medium. At 24 and 48 h ...
Alkyne-tagged SERS nanoprobe for understanding Cu+ and Cu2+ conversion in cuproptosis processes
Zhang, Mei, Liu et al
Nat Commun (2024) 15 (1), 3246 DOI: 10.1038/s41467-024-47549-1
Zhang, Mei, Liu et al
Nat Commun (2024) 15 (1), 3246 DOI: 10.1038/s41467-024-47549-1
Cited by 53
AI Summary: OriGene products contributed to this study by providing primary antibodies against synthesis of cytochrome c oxidase 1 (SCO1, TA381301) and dihydrolipoamide S-acetyltransferase (DLAT, TA350579), which were used to analyze protein expression related to copper metabolism and cuproptosis processes.
Snippet: ther was incubated respectively with the primary antibody against cytochrome C oxidase 17 (Cox 17, 1: 2000, Abcam ab69611), primary antibody against synthesis of cytochrome c oxidase 1 (SCO1, 1:2000, OriGene Technologies, TA381301), primary antibody against dihydrolipoamide S-acetyltransferase (DLAT, 1:1000, OriGene Technologies, TA350579), and primary antibody against β-actin (1:5000, Abcam ab82
Showing 1-2 of 2 papers.
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