Glycerol 3 Phosphate Dehydrogenase (GPD1) (NM_005276) Human Recombinant Protein

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SKU
TP306538
Recombinant protein of human glycerol-3-phosphate dehydrogenase 1 (soluble) (GPD1), 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC206538 protein sequence
Red=Cloning site Green=Tags(s)

MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLP
PNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERL
GIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVG
AGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFART
GKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 37.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_005267
Locus ID 2819
UniProt ID P21695
Cytogenetics 12q13.12
RefSeq Size 3083
RefSeq ORF 1047
Synonyms GPD-C; GPDH-C; HTGTI
Summary This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Mar 2012
Protein Categories Intracellular Proteins
Protein Pathways Glycerophospholipid metabolism
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Other "Glycerol 3 Phosphate Dehydrogenase" proteins (4)
SKU Description Size Price
PH306538 GPD1 MS Standard C13 and N15-labeled recombinant protein (NP_005267) 10 ug
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LC417409 Lyophilized GPD1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417409 Transient overexpression lysate of glycerol-3-phosphate dehydrogenase 1 (soluble) (GPD1) (as WB positive control) 100 ug
$436.00
TP720715 Purified recombinant protein of Human glycerol-3-phosphate dehydrogenase 1 (soluble) (GPD1) 10 ug
$340.00

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