Dynamin 1 (DNM1) (NM_001005336) Human Recombinant Protein

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SKU
TP306284
Recombinant protein of human dynamin 1 (DNM1), transcript variant 2, 20 µg
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC206284 representing NM_001005336
Red=Cloning site Green=Tags(s)

MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV
LQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM
TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALEVAKEVDPQGQRTIGVITKLDLM
DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV
LNQQLTDHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ
IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKI
REPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH
EDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKE
KKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERV
GDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSE
LLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPA
GRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSAMGGAPPVPSRPGASPDPFGPPPQVPSRPNRAP
PGVPRITISDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 95.9 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001005336
Locus ID 1759
UniProt ID Q05193
Cytogenetics 9q34.11
RefSeq Size 3253
RefSeq ORF 2553
Synonyms DEE31; DNM; EIEE31
Summary This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins, Nervous system Diseases
Protein Families Druggable Genome
Protein Pathways Endocytosis, Fc gamma R-mediated phagocytosis
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Documents
Resources
Other "Dynamin 1" proteins (5)
SKU Description Size Price
PH306284 DNM1 MS Standard C13 and N15-labeled recombinant protein (NP_001005336) 10 ug
$3,360.00
LC418003 Lyophilized DNM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423862 Lyophilized DNM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418003 Transient overexpression lysate of dynamin 1 (DNM1), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY423862 Transient overexpression lysate of dynamin 1 (DNM1), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity
Barber, Goldschmidt, Ma et al
Front Synaptic Neurosci (2022) 14, 855673 DOI: 10.3389/fnsyn.2022.855673
Cited by 7
AI Summary: OriGene supplied purified proteins (Camk2a [TP318186], Hspa8 [TP302209], Synaptogyrin-1 [TP300562], Dynamin-1 [TP306284], and Munc18-1 [TP318471]) that were used to assay their interactions with DGKθ and their effects on its kinase activity in the study.
Snippet: Goat anti-Rabbit Alexa Fluor 568 Invitrogen A11036 1:500 – ------------------------- Purified Proteins Source Product Number ------------------------- Camk2a Origene TP318186 Hspa8 Origene TP302209 Synaptogyrin-1 Origene TP300562 Dynamin-1 Origene TP306284 Munc18-1 Origene TP318471 Syntaxin-7 NovoPro Biosci
FigureFigureFigure
Showing 1-1 of 1 papers.
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