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Epac2 (RAPGEF4) (NM_007023) Human Recombinant Protein

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SKU
TP305335
Recombinant protein of human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, 20 µg
Size:
20 ug 1 mg 100 ug
  $737.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC205335 protein sequence
Red=Cloning site Green=Tags(s)

MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITL
FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQ
KDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKIL
RNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHF
QDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEE
LLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDD
FGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQG
NSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQ
GTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIV
KQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDPTYTTIRVPVATSVKEVISAVA
DKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFMTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFEL
MSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKR
VQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYR
LTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNH
QDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 115.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_008954
Locus ID 11069
UniProt ID Q8WZA2
Cytogenetics 2q31.1
RefSeq Size 4361
RefSeq ORF 3033
Synonyms CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
Summary Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
Protein Pathways Leukocyte transendothelial migration
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Other "Epac2" proteins (3)
SKU Description Size Price
PH305335 RAPGEF4 MS Standard C13 and N15-labeled recombinant protein (NP_008954) 10 ug
$3,360.00
LC416236 Lyophilized RAPGEF4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY416236 Transient overexpression lysate of Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1 (as WB positive control) 100 ug
$436.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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