Caspase 3 (CASP3) (NM_032991) Human Recombinant Protein
SKU
TP304444
Recombinant protein of human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, 20 µg
$867.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 31.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_116786 |
| Locus ID | 836 |
| UniProt ID | P42574 |
| Cytogenetics | 4q35.1 |
| RefSeq Size | 2522 |
| RefSeq ORF | 831 |
| Synonyms | CPP32; CPP32B; SCA-1 |
| Summary | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. provided by RefSeq, Aug 2017 |
| Protein Categories | Enzyme: Peptidases, Intracellular Proteins |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protease |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Caspase 3" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH304444 | CASP3 MS Standard C13 and N15-labeled recombinant protein (NP_116786) | 10 ug |
$3,360.00
|
|
| PH323662 | CASP3 MS Standard C13 and N15-labeled recombinant protein (NP_004337) | 10 ug |
$3,360.00
|
|
| LC418048 | Lyophilized CASP3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY418048 | Transient overexpression lysate of caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha (as WB positive control) | 100 ug |
$436.00
|
|
| TP323662 | Recombinant protein of human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant alpha, 20 µg | 20 ug |
$867.00
|
|
Citations
Endogenous Hydrogen Sulfide Persulfidates Caspase-3 at Cysteine 163 to Inhibit Doxorubicin-Induced Cardiomyocyte Apoptosis
Ye, Li, Lv et al
Oxid Med Cell Longev (2022) 2022, 6153772 DOI: 10.1155/2022/6153772
Ye, Li, Lv et al
Oxid Med Cell Longev (2022) 2022, 6153772 DOI: 10.1155/2022/6153772
Cited by 25
AI Summary: OriGene’s recombinant human caspase-3 protein was used in this study to investigate how hydrogen sulfide (H2S) directly persulfidates caspase-3, demonstrating that this modification inhibits caspase-3 activity and protects cardiomyocytes from doxorubicin-induced apoptosis.
Snippet: ee SDS loading buffer, boiled, centrifuged at 5000 g to collect the persulfidated proteins, and loaded onto 10% SDS–PAGE to analyze caspase-3 persulfidation. Recombinant human caspase-3 protein (Origene, Rockville, USA) was divided into control, NaHS, and NaHS+DTT groups. In the NaHS group, 0.15 μg of caspase-3 protein was incubated with 100 μmol/L NaHS for 30 min at 37°C, while in t
Chemoproteomics-Enabled Covalent Ligand Screening Reveals a Thioredoxin-Caspase 3 Interaction Disruptor That Impairs Breast Cancer Pathogenicity
Anderson, To, Olzmann et al
ACS Chem. Biol. (2017)
Anderson, To, Olzmann et al
ACS Chem. Biol. (2017)
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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