GBE1 (NM_000158) Human Recombinant Protein

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SKU
TP304152
Recombinant protein of human glucan (1,4-alpha-), branching enzyme 1 (GBE1), 20 µg
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC204152 protein sequence
Red=Cloning site Green=Tags(s)

MAAPMTPAARPEDYEAALNAALADVPELARLLEIDPYLKPYAVDFQRRYKQFSQILKNIGENEGGIDKFS
RGYESFGVHRCADGGLYCKEWAPGAEGVFLTGDFNGWNPFSYPYKKLDYGKWELYIPPKQNKSVLVPHGS
KLKVVITSKSGEILYRISPWAKYVVREGDNVNYDWIHWDPEHSYEFKHSRPKKPRSLRIYESHVGISSHE
GKVASYKHFTCNVLPRIKGLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGSPEELQELVDTAHSMG
IIVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRGTHDLWDSRLFAYSSWEVLRFLLSNIRWWLEEYR
FDGFRFDGVTSMLYHHHGVGQGFSGDYSEYFGLQVDEDALTYLMLANHLVHTLCPDSITIAEDVSGMPAL
CSPISQGGGGFDYRLAMAIPDKWIQLLKEFKDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSL
AFWLMDAEMYTNMSVLTPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESY
HYARRQFHLTDDDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEKHEGNKIIAFERAGLLFIFNFHPS
KSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDHSTDFFSEAFEHNGRPYSLLVYIPSRVALILQNVDL
PN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 80.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_000149
Locus ID 2632
UniProt ID Q04446
Cytogenetics 3p12.2
RefSeq Size 3118
RefSeq ORF 2106
Synonyms APBD; GBE; GSD4
Summary The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Starch and sucrose metabolism
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Documents
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Other "GBE1" proteins (3)
SKU Description Size Price
PH304152 GBE1 MS Standard C13 and N15-labeled recombinant protein (NP_000149) 10 ug
$3,360.00
LC400056 Lyophilized GBE1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400056 Transient overexpression lysate of glucan (1,4-alpha-), branching enzyme 1 (GBE1) (as WB positive control) 100 ug
$436.00

file-alt Citations

Triacylglycerol mimetics regulate membrane interactions of glycogen branching enzyme: implications for therapy
Alvarez, Casas, López et al
J. Lipid Res. (2017)
AI Summary: OriGene contributed purified commercial GBE1 protein and an anti-GBE1 antibody, which were used in the study to analyze GBE1 activity and protein interactions.
Snippet: Purified commercial GBE1 protein and the anti-GBE1 antibody were supplied by OriGene Technologies, Inc. … immunoreactive bands), consisting of four points of different protein content prepared from a commercial GBE1 protein batch (Origene ... … Purified commercial GBE1 protein and the anti-GBE1 antibody were supplied by OriGene Technologies, Inc. … immunoreactive bands), consisting of four points of different protein content prepared from a commercial GBE1 protein batch (Origene ... …
Showing 1-1 of 1 papers.
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