PGLS (NM_012088) Human Recombinant Protein

CAT#: TP304132

Recombinant protein of human 6-phosphogluconolactonase (PGLS), 20 µg

Product Images

Coomassie blue staining of purified PGLS protein (Cat# TP304132). The protein was produced from HEK293T cells transfected with PGLS cDNA clone (Cat# RC204132) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC204132 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASL
  ARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIP
  VFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAA
  VLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 27.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_036220
• Locus ID: 25796
• UniProt ID: O95336, A0A0K0K1K7
• Cytogenetics: 19p13.11
• Refseq Size: 1043
• Refseq ORF: 774
• Synonyms: 6PGL; HEL-S-304
• Summary: Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.[UniProtKB/Swiss-Prot Function]
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway