PUS7 (NM_019042) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 74.9 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_061915 |
| Locus ID | 54517 |
| UniProt ID | Q96PZ0 |
| Cytogenetics | 7q22.3 |
| RefSeq Size | 3484 |
| RefSeq ORF | 1983 |
| Synonyms | IDDABS |
| Summary | Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "PUS7" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303902 | PUS7 MS Standard C13 and N15-labeled recombinant protein (NP_061915) | 10 ug |
$3,360.00
|
|
| LC412749 | Lyophilized PUS7 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY412749 | Transient overexpression lysate of pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
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