beta Actin (ACTB) (NM_001101) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 41.6 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_001092 |
| Locus ID | 60 |
| UniProt ID | P60709 |
| Cytogenetics | 7p22.1 |
| RefSeq Size | 1793 |
| RefSeq ORF | 1125 |
| Synonyms | BRWS1; PS1TP5BP1 |
| Summary | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. provided by RefSeq, Aug 2017 |
| Protein Categories | ADC Targets, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "beta Actin" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303643 | ACTB MS Standard C13 and N15-labeled recombinant protein (NP_001092) | 10 ug |
$3,360.00
|
|
| LC420311 | Lyophilized ACTB HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY420311 | Transient overexpression lysate of actin, beta (ACTB) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
High throughput-screening of native herbal compounds identifies taccaoside A as a cytotoxic compound that mediates RAS signaling in cancer stem cells
Yang, Dai, Zhu et al
Phytomedicine (2022) 108, 154492 DOI: 10.1016/j.phymed.2022.154492
Yang, Dai, Zhu et al
Phytomedicine (2022) 108, 154492 DOI: 10.1016/j.phymed.2022.154492
Cited by 9
AI Summary: OriGene's β-actin (TP303643) was used as a control protein in surface plasmon resonance (SPR) assays to assess the specificity of compound interactions in this study.
Snippet: ), and β-actin (TP303643, OriGene). Each protein species was serially diluted to final concentrations of 0.2 μM, 0.4 μM, 0.8 μM, 1.6 μM and 3.2 μM prior to SPR interaction
Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway
Lu, Zhang, Zhang et al
Oxid Med Cell Longev (2021) 2021, 8869908 DOI: 10.1155/2021/8869908
Lu, Zhang, Zhang et al
Oxid Med Cell Longev (2021) 2021, 8869908 DOI: 10.1155/2021/8869908
Cited by 12
AI Summary: OriGene Technologies’ β-actin antibody was used as a loading control in Western blot experiments to normalize protein expression levels in the study investigating how BOXes induce neuronal oxidative stress via the Nrf2 pathway.
Snippet: (Chicago, IL, USA);Nrf2 (WB, 1 : 1000, IF, 1:200) and Gsta3 (1:500) from Abcam (Cambridge, MA, USA);SRXN1 (1 : 500) from Bioss (Beijing, China);Caspase 3 (WB, 1:1000) from Abmart (Shanghai, China);and β-actin (WB, 1:2000) from OriGene Technologies, Inc
DCAF1 regulates Treg senescence via the ROS axis during immunological aging
Guo, Wang, Wu et al
J Clin Invest (2020) 130 (11), 5893-5908 DOI: 10.1172/JCI136466
Guo, Wang, Wu et al
J Clin Invest (2020) 130 (11), 5893-5908 DOI: 10.1172/JCI136466
AI Summary: OriGene products, specifically the mouse GSTP1 shRNA plasmid (TL501412) and mouse GSTP1 overexpression plasmid (MR204939), were used in this study to manipulate GSTP1 expression in Treg cells, enabling the authors to investigate the role of GSTP1 in regulating Treg senescence through the ROS axis.
The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase
Seervai, Jangid, Karki et al
Sci Adv (2020) 6 (40) DOI: 10.1126/sciadv.abb7854
Seervai, Jangid, Karki et al
Sci Adv (2020) 6 (40) DOI: 10.1126/sciadv.abb7854
Cited by 54
SETD2 is an actin lysine methyltransferase
Seervai, Jangid, Karki et al
bioRxiv (2020)
Seervai, Jangid, Karki et al
bioRxiv (2020)
Cited by 1
Snippet: gizzard smooth muscle actin (Cytoskeleton, AS99), beta actin (NM_001101) human recombinant protein (Origene, TP303643), beta actin (NM_001101) human recombinant protein (Origene, TP720518). In Vitro Methyltransferase Assays;- gizzard smooth muscle actin (Cytoskeleton, AS99), beta actin (NM_001101) human recombinant protein (Origene, TP303643), beta actin (NM_001101) human recombinant protein (Origene, TP720518). …
Myeloid-Derived Lymphatic Endothelial Cell Progenitors Significantly Contribute to Lymphatic Metastasis in Clinical Breast Cancer
Volk-Draper, Patel, Bhattarai et al
Am. J. Pathol. (2019) 189 (11), 2269-2292 DOI: 10.1016/j.ajpath.2019.07.006
Volk-Draper, Patel, Bhattarai et al
Am. J. Pathol. (2019) 189 (11), 2269-2292 DOI: 10.1016/j.ajpath.2019.07.006
AI Summary: OriGene products contributed to this paper by providing plasmids containing full-length cDNA for human and mouse VEGFC and β-actin, which were used as standards in quantitative analyses.
Snippet: Skip to article;Total RNA was isolated from orthotopic MDA-MB-231 tumors and converted to cDNA using Superscript ViLo kit (Life Technology). Species specificity of primers for VEGFC and β-actin (Table 2) was tested using human and mouse universal cDNA templates followed by agarose gel analysis of products. Custom TaqMan primer:probe sets were designed for each species-specific primer set and synth...
Showing 1-6 of 10 papers.
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