Nuclear Factor Erythroid Derived 2 (NFE2) (NM_006163) Human Recombinant Protein

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SKU
TP302924
Recombinant protein of human nuclear factor (erythroid-derived 2), 45kDa (NFE2), 20 µg
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC202924 protein sequence
Red=Cloning site Green=Tags(s)

MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSI
HPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQE
DPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSG
PVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAA
QNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEY
ALQQAADGTIFLVPRGTKMEATD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 41.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_006154
Locus ID 4778
UniProt ID Q16621
Cytogenetics 12q13.13
RefSeq Size 1697
RefSeq ORF 1119
Synonyms NF-E2; p45
Summary Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins, Transciption Factors
Protein Families Transcription Factors
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Other "Nuclear Factor Erythroid Derived 2" proteins (6)
SKU Description Size Price
PH302924 NFE2 MS Standard C13 and N15-labeled recombinant protein (NP_006154) 10 ug
$3,360.00
LC401856 Lyophilized NFE2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427772 Lyophilized NFE2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401856 Transient overexpression lysate of nuclear factor (erythroid-derived 2), 45kDa (NFE2), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY427772 Transient overexpression lysate of nuclear factor (erythroid-derived 2), 45kDa (NFE2), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP761189 Purified recombinant protein of Human nuclear factor (erythroid-derived 2), 45kDa (NFE2), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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