NQO2 (NM_000904) Human Recombinant Protein

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SKU
TP302889
Recombinant protein of human NAD(P)H dehydrogenase, quinone 2 (NQO2), 20 µg
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC202889 protein sequence
Red=Cloning site Green=Tags(s)

MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGV
ETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQG
KLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQ
RLQTIWKEEPIPCTAHWHFGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 25.7 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_000895
Locus ID 4835
UniProt ID P16083
Cytogenetics 6p25.2
RefSeq Size 1272
RefSeq ORF 693
Synonyms DHQV; DIA6; NMOR2; QR2
Summary This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
Protein Categories Cancer: Gynecologic, Intracellular Proteins
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Other "NQO2" proteins (3)
SKU Description Size Price
PH302889 NQO2 MS Standard C13 and N15-labeled recombinant protein (NP_000895) 10 ug
$3,360.00
LC424463 Lyophilized NQO2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY424463 Transient overexpression lysate of NAD(P)H dehydrogenase, quinone 2 (NQO2) (as WB positive control) 100 ug
$436.00

file-alt Citations

Reduction and scavenging of chemically reactive drug metabolites by NAD(P)H:quinone oxidoreductase 1 and NRH:quinone oxidoreductase 2 and variability in hepatic concentrations
den Braver-Sewradj, den Braver, Toorneman et al
Chem. Res. Toxicol. (2017)
AI Summary: OriGene’s human NQO2 cDNA ORF clone (catalog number SC319150) was used to generate recombinant NQO2 for expression and purification, enabling the study of its enzymatic activity in reducing and scavenging reactive drug metabolites in human liver.
Snippet: Expression and purification of recombinant human NQO2 NQO2 DNA was obtained by PCR amplification from the human cDNA ORF clone (SC319150, obtained from OriGene Technologies, Rockville, MD) using the forward primer 5'- GCAACATATGGCAGGTAAGAAAGTAC View as HTML;ADVERTISEMENT;Expression and purification of recombinant human NQO2 NQO2 DNA was obtained by PCR amplification from the human cDNA ORF clone (SC319150, obtained from OriGene Technologies, Rockville, MD) using the forward primer 5'- GCAACATATGGCAGGTAAGAAAGTAC
Showing 1-1 of 1 papers.
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