RIP2 (RIPK2) (NM_003821) Human Recombinant Protein

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SKU
TP302530
Recombinant protein of human receptor-interacting serine-threonine kinase 2 (RIPK2), 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC202530 protein sequence
Red=Cloning site Green=Tags(s)

MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEIL
HKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTP
PLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKH
DIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDER
PSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQ
LHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIIN
PLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQL
LDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 61 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_003812
Locus ID 8767
UniProt ID O43353
Cytogenetics 8q21.3
RefSeq Size 2588
RefSeq ORF 1620
Synonyms CARD3; CARDIAK; CCK; GIG30; RICK; RIP2
Summary This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. provided by RefSeq, Jul 2008
Protein Categories Chemokines, Cytokines, Enzyme: Kinases, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Neurotrophin signaling pathway, NOD-like receptor signaling pathway
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Other "RIP2" proteins (3)
SKU Description Size Price
PH302530 RIPK2 MS Standard C13 and N15-labeled recombinant protein (NP_003812) 10 ug
$3,360.00
LC418408 Lyophilized RIPK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418408 Transient overexpression lysate of receptor-interacting serine-threonine kinase 2 (RIPK2) (as WB positive control) 100 ug
$436.00

file-alt Citations

A Chemical Biology Approach to the Cell Type Selective Killing of Glioblastoma Tumor Cells
West
Thesis (2024)
Snippet: 1.0 μg recombinant full-length (TP302530, Origene) RIPK2 was incubated with cRIPGBM-PAP (1 µM) with or without varying concentrations of YM155 in 100 µL PBS containing 0.1% TWEEN-20. After incubation at room temperature for 15 minutes, samples were irradiated for 45 minutes using a handheld UV lamp (254nm)
Showing 1-1 of 1 papers.
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