SNAP25 (NM_003081) Human Recombinant Protein
SKU
TP302068
Recombinant protein of human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1, 20 µg
$737.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 23.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | MS digestion standard (PMID: 25418885) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_003072 |
| Locus ID | 6616 |
| UniProt ID | P60880 |
| Cytogenetics | 20p12.2 |
| RefSeq Size | 2069 |
| RefSeq ORF | 618 |
| Synonyms | bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP |
| Summary | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. provided by RefSeq, Jul 2008 |
| Protein Categories | Growth Factors, Intracellular Proteins, Nervous system Diseases |
| Protein Families | Druggable Genome |
| Protein Pathways | SNARE interactions in vesicular transport |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SNAP25" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH302068 | SNAP25 MS Standard C13 and N15-labeled recombinant protein (NP_003072) | 10 ug |
$3,360.00
|
|
| PH312596 | SNAP25 MS Standard C13 and N15-labeled recombinant protein (NP_570824) | 10 ug |
$3,360.00
|
|
| LC408904 | Lyophilized SNAP25 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC418912 | Lyophilized SNAP25 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY408904 | Transient overexpression lysate of synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY418912 | Transient overexpression lysate of synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP312596 | Recombinant protein of human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
Interaction of Synaptosomal-Associated Protein 25 with Neutral Sphingomyelinase 2: Functional Impact on the Sphingomyelin Pathway
Won, Jeon, Kim et al
Neuroscience (2019) 427, 1-15 DOI: 10.1016/j.neuroscience.2019.08.015
Won, Jeon, Kim et al
Neuroscience (2019) 427, 1-15 DOI: 10.1016/j.neuroscience.2019.08.015
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons
Maidorn, Olichon, Rizzoli et al
MAbs (2018)
Maidorn, Olichon, Rizzoli et al
MAbs (2018)
AI Summary: OriGene products, specifically homolog proteins of SNAP-25 and Syntaxin 1A, were used for specificity and epitope mapping analyses to validate the nanobodies developed in this study.
Snippet: For specificity and epitope mapping analysis in Figure 2, dot blots were performed as mentioned above, but detection was performed using directly conjugated nanobodies to Atto647N fluorophore and read in the Amersham Imager 600 (GE Healthcare). Homolog proteins of SNAP-25 and Syntaxin 1A were all purchased from OriGene, Rockville, MD, USA and truncated versions were clone and produced as explained above
SNAP-25 is a promising novel cerebrospinal fluid biomarker for synapse degeneration in Alzheimer's disease
Brinkmalm, Brinkmalm, Honer et al
Mol Neurodegener (2014) 9 (1), 53 DOI: 10.1186/1750-1326-9-53
Brinkmalm, Brinkmalm, Honer et al
Mol Neurodegener (2014) 9 (1), 53 DOI: 10.1186/1750-1326-9-53
Cited by 303
AI Summary: OriGene's recombinant SNAP-25 standard protein was used as a reference in immunoprecipitation assays to quantify SNAP-25 levels in cerebrospinal fluid, enabling the development and validation of a sensitive biomarker assay for synapse degeneration in Alzheimer's disease.
Snippet: 25]. The SMI81 antibody recognizes an epitope containing the N-terminally acetylated first 11 amino acids of brain SNAP-25 [25]. Recombinant standard protein of SNAP-25 was purchased from Origene. Immunoprecipitation ... Cite SaveSaving
Targeting synaptic pathology with a novel affinity mass spectrometry approach
Brinkmalm, Brinkmalm, Honer et al
Mol. Cell Proteomics (2014)
Brinkmalm, Brinkmalm, Honer et al
Mol. Cell Proteomics (2014)
AI Summary: OriGene contributed to the study by providing the recombinant standard protein of SNAP-25, which was used as a reference in the affinity mass spectrometry analysis of synaptic proteins.
Snippet: anti-SNAP-25 antibody was raised in rabbit accordingly to (25). Recombinant standard protein of SNAP-25 was purchased from Origene. Immunoprecipitation The immunoprecipitation method of brain tissue extracts was performed according to Öhrfelt et al. ... Cite SaveSaving
Showing 1-4 of 4 papers.
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