SMAD4 (NM_005359) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 60.3 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Co-immunoprecipitation (PMID: 26772959) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_005350 |
| Locus ID | 4089 |
| UniProt ID | Q13485 |
| Cytogenetics | 18q21.2 |
| RefSeq Size | 3220 |
| RefSeq ORF | 1656 |
| Synonyms | DPC4; JIP; MADH4; MYHRS |
| Summary | This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. provided by RefSeq, Aug 2017 |
| Protein Categories | Cancer: Gastrointestinal, Growth Factors, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SMAD4" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301828 | SMAD4 MS Standard C13 and N15-labeled recombinant protein (NP_005350) | 10 ug |
$3,360.00
|
|
| LC401648 | Lyophilized SMAD4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401648 | Transient overexpression lysate of SMAD family member 4 (SMAD4) (as WB positive control) | 100 ug |
$436.00
|
|
| TP762318 | Purified recombinant protein of Human SMAD family member 4 (SMAD4), Ala152-Tyr322, with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$260.00
|
|
| TP762677 | Purified recombinant protein of Human SMAD family member 4 (SMAD4), 1Met-195Tyr, with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$270.00
|
|
Citations
M10, a caspase cleavage product of the hepatocyte growth factor receptor, interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo
Atanelishvili, Shirai, Akter et al
Transl Res (2015)
Atanelishvili, Shirai, Akter et al
Transl Res (2015)
Cited by 11
AI Summary: OriGene products, including recombinant human Smad2 (NM_005901) and Smad4 (NM_005359) with C-terminal MYC/DDK tags, as well as the anti-DDK antibody, were used in this study to investigate the interaction between the M10 peptide and Smad proteins, contributing to the demonstration of M10's antifibrotic properties.
Showing 1-1 of 1 papers.
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