RAD18 (NM_020165) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 56 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | In vitro protein binding assay (PMID: 25961918) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_064550 |
| Locus ID | 56852 |
| UniProt ID | Q9NS91 |
| Cytogenetics | 3p25.3 |
| RefSeq Size | 5739 |
| RefSeq ORF | 1485 |
| Synonyms | RNF73 |
| Summary | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "RAD18" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301700 | RAD18 MS Standard C13 and N15-labeled recombinant protein (NP_064550) | 10 ug |
$3,360.00
|
|
| LC402768 | Lyophilized RAD18 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402768 | Transient overexpression lysate of RAD18 homolog (S. cerevisiae) (RAD18) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
USP7 is essential for maintaining Rad18 stability and DNA damage tolerance
Zlatanou, Sabbioneda, Miller et al
Oncogene (2015)
Zlatanou, Sabbioneda, Miller et al
Oncogene (2015)
Cited by 77
AI Summary: In this study, OriGene's purified Rad18 protein was used in immunoprecipitation experiments to investigate the direct interaction between Rad18 and USP7, helping to elucidate USP7's role in regulating Rad18 stability and DNA damage tolerance.
Snippet: Immunoprecipitations using purified proteins were carried out as follows: 500 ng of purified USP7 (BostonBiochem, R&D Systems Europe Ltd, Abingdon, UK) and Rad18 (ORIGENE, Insight Biotechnology, Middlesex, UK) were mixed for 2 h at 4 °C in a 500 μl reaction ... Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
