MIIP (NM_021933) Human Recombinant Protein

Snippet: Migration and invasion inhibitory protein (MIIP) has been identified as a tumor suppressor in various cancer types. Although MIIP is reported to exert tumor suppressive functions by repressing proliferation and metastasis of cancer cells, the detailed mechanism is poorly understood. In the present study, we found MIIP is a favorable indicator of prognosis in triple-negative breast cancer. MIIP could inhibit tumor angiogenesis, proliferation, and metastasis of triple-negative breast cancer cells in vivo and in vitro. Mechanistically, MIIP directly interacted with ITGB3 and suppressed its downstream signaling. … More importantly, we found RGD motif is essential for MIIP binding with ITGB3 and executing efficient tumor-suppressing effect. Our findings unravel a novel mechanism by which MIIP suppresses tumorigenesis in triple-negative breast cancer, and MIIP is thus a promising molecular biomarker or therapeutic target for the disease. 2022. …

Snippet: extract. To determine whether Topo II activity was regulated by MIIP specifically,we used a mixture of 0.25 IU of Topo II (TopoGEN) and different doses of purified MIIP protein(OriGene, Rockville, MD) to replace the nuclear extracts. This reaction was kept at 37 C for ... Cite SaveSaving