DDOST (NM_005216) Human Recombinant Protein

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SKU
TP300672
Recombinant protein of human dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST), 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC200672 protein sequence
Red=Cloning site Green=Tags(s)

MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVLLDNLNVRETHSLFFRSLKDR
GFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRE
LGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPL
VLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGS
QRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDG
DDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYER
FIPSAYPYYASAFSMMLGLFIFSIVFLHMKEKEKSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 46.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_005207
Locus ID 1650
UniProt ID P39656
Cytogenetics 1p36.12
RefSeq Size 2144
RefSeq ORF 1368
Synonyms AGER1; CDG1R; GATD6; OKSWcl45; OST; OST48; WBP1
Summary This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. provided by RefSeq, Jul 2008
Protein Categories Cytokines, Intracellular Proteins, Membrane Proteins
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
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Other "DDOST" proteins (3)
SKU Description Size Price
PH300672 DDOST MS Standard C13 and N15-labeled recombinant protein (NP_005207) 10 ug
$3,360.00
LC417440 Lyophilized DDOST HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417440 Transient overexpression lysate of dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST) (as WB positive control) 100 ug
$436.00

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