Neuraminidase (NEU1) (NM_000434) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 40.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Cell treatment (PMID: 29118338) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_000425 |
| Locus ID | 4758 |
| UniProt ID | Q99519 |
| Cytogenetics | 6p21.33 |
| RefSeq Size | 2088 |
| RefSeq ORF | 1245 |
| Synonyms | NANH; NEU; SIAL1 |
| Summary | The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Lysosome, Other glycan degradation, Sphingolipid metabolism |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Neuraminidase" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH300386 | NEU1 MS Standard C13 and N15-labeled recombinant protein (NP_000425) | 10 ug |
$3,360.00
|
|
| LC424720 | Lyophilized NEU1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY424720 | Transient overexpression lysate of sialidase 1 (lysosomal sialidase) (NEU1) (as WB positive control) | 100 ug |
$436.00
|
|
| TP721041 | Purified recombinant protein of Human sialidase 1 (lysosomal sialidase) (NEU1) | 10 ug |
$340.00
|
|
Citations
The extracellular sialidase NEU3 primes neutrophils
Kirolos, Pilling, Gomer
J Leukoc Biol (2022)
Kirolos, Pilling, Gomer
J Leukoc Biol (2022)
Endolysosomal N-glycan processing is critical to attain the most active form of the enzyme acid alpha-glucosidase
Selvan, Mehta, Venkateswaran et al
J Biol Chem (2021) 296, 100769 DOI: 10.1016/j.jbc.2021.100769
Selvan, Mehta, Venkateswaran et al
J Biol Chem (2021) 296, 100769 DOI: 10.1016/j.jbc.2021.100769
Cited by 21
AI Summary: In this study, OriGene’s GAA (OriGene, Cat# TP301482) and GAA cDNA clone (OriGene, Cat# RC218307) were used to investigate how specific glycan and proteolytic processing steps affect the activation and efficacy of acid alpha-glucosidase, informing strategies for improved enzyme replacement therapy in Pompe disease.
Attenuated pulmonary fibrosis in sialidase-3 knockout (Neu3-/-) mice
Karhadkar, Chen, Gomer
Am J Physiol Lung Cell Mol Physiol (2020) 318 (1), L165-L179 DOI: 10.1152/ajplung.00275.2019
Karhadkar, Chen, Gomer
Am J Physiol Lung Cell Mol Physiol (2020) 318 (1), L165-L179 DOI: 10.1152/ajplung.00275.2019
Cited by 34
Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model
Karhadkar, Pilling, Cox et al
Sci Rep (2017) 7 (1), 15069 DOI: 10.1038/s41598-017-15198-8
Karhadkar, Pilling, Cox et al
Sci Rep (2017) 7 (1), 15069 DOI: 10.1038/s41598-017-15198-8
AI Summary: In this study, OriGene’s recombinant human NEU1, NEU2, NEU3, and NEU4 proteins were used to investigate the effects of sialidases on immune cell activity and fibrosis development in vitro.
Snippet: Where indicated, recombinant 10 ng/ml human TGF-β1 (Peprotech, Rocky Hill, NJ), 200 ng/ml recombinant human NEU1, 2, 3, or 4 (Origene, Rockville, MD), or 2 µg/ml human SAP (EMD Millipore, Billerica, MA;with the azide removed by centrifugal filters following86) were added to cells. Fibrocytes were counted as previously described29,84
Showing 1-4 of 4 papers.
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