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Nkx2.2 (NKX2-2) (NM_002509) Human Over-expression Lysate

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SKU
LY419283
Transient overexpression lysate of NK2 homeobox 2 (NKX2-2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210419
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002509, NP_002500
Synonyms NKX2.2; NKX2B
Predicted MW 30 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002500
Locus ID 4821
Cytogenetics 20p11.22
Protein Families WB
Protein Pathways Maturity onset diabetes of the young
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Other "Nkx2.2" proteins (2)
SKU Description Size Price
LC419283 Lyophilized NKX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP760219 Recombinant protein of human NK2 homeobox 2 (NKX2-2), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Deregulated expression of NKL homeobox genes in T-cell lymphomas
Nagel, Pommerenke, MacLeod et al
Oncotarget (2019) 10 (35), 3227-3247 DOI: 10.18632/oncotarget.26929
Snippet: … Mutations consisting in both cell lines were generated early in the course of tumorigenesis and include i(7q) targeting AUTS2, loss-of-function mutation of SETD2, deletion of both alleles of CDKN2A, and deletion of one allele of SLFN13. Mutations consisting in just one cell line r;Transfections of gene expression construct for NKX2-2 (cloned in vector pCMV6-XL4 and obtained from Origene, Wiesbaden, Germany) into NIH-3T3 cells was performed using SuperFect Transfection Reagent (Qiagen)
FigureFigureFigure
Showing 1-1 of 1 papers.
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