FATP2 (SLC27A2) (NM_003645) Human Over-expression Lysate
SKU
LY418528
Transient overexpression lysate of solute carrier family 27 (fatty acid transporter), member 2 (SLC27A2), transcript variant 1 (as WB positive control)
$665.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221033 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003645, NP_003636 |
| Synonyms | ACSVL1; FACVL1; FATP2; hFACVL1; HsT17226; VLACS; VLCS |
| Predicted MW | 70.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_003636 |
| Locus ID | 11001 |
| Cytogenetics | 15q21.2 |
| Protein Families | WB |
| Protein Pathways | PPAR signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "FATP2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH321033 | SLC27A2 MS Standard C13 and N15-labeled recombinant protein (NP_003636) | 10 ug |
$3,360.00
|
|
| LC418528 | Lyophilized SLC27A2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP321033 | Recombinant protein of human solute carrier family 27 (fatty acid transporter), member 2 (SLC27A2), 20 µg | 20 ug |
$867.00
|
|
Citations
MYCN-driven fatty acid uptake is a metabolic vulnerability in neuroblastoma
Tao, Mohammad, Milazzo et al
Nat Commun (2022) 13 (1), 3728 DOI: 10.1038/s41467-022-31331-2
Tao, Mohammad, Milazzo et al
Nat Commun (2022) 13 (1), 3728 DOI: 10.1038/s41467-022-31331-2
Cited by 81
AI Summary: OriGene’s FATP2 cDNA was used to generate Tet-inducible overexpression models in neuroblastoma cells, enabling the study of how increased FATP2 expression—driven by MYCN—affects fatty acid uptake and tumor cell survival.
Snippet: 98610, GE Healthcare Dharmacon, CO, USA) were used in LAN5 cells. V3LHS_398610 (with better KD efficiency) was used in IMR32 and SK-N-AS cells. FATP1 or FATP2 overexpression: FATP1 (C-BASS) or FATP2 (OriGene) cDNAs were cloned into pINDUCER20, a Tet-inducible lentiviral vector for ORF expression. Second generation lentiviruses were prepared as previously described64. Mouse models In vivo studies
Showing 1-1 of 1 papers.
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