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Tau tubulin kinase 2 (TTBK2) (NM_173500) Human Over-expression Lysate

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SKU
LY406582
Transient overexpression lysate of tau tubulin kinase 2 (TTBK2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209688
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_173500, NP_775771
Synonyms SCA11; TTBK
Predicted MW 137.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_775771
Locus ID 146057
Cytogenetics 15q15.2
Protein Families WB
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Other "Tau tubulin kinase 2" proteins (1)
SKU Description Size Price
LC406582 Lyophilized TTBK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

The Tau Tubulin Kinases TTBK1/2 Promote Accumulation of Pathological TDP-43
Liachko, McMillan, Strovas et al
PLoS Genet. (2014) 10 (12), e1004803 DOI: 10.1371/journal.pgen.1004803
Cited by 130
AI Summary: OriGene’s recombinant TTBK2 enzyme (catalog #LY406582) was used in in vitro kinase assays to confirm enzyme activity by phosphorylating tau, supporting the study’s investigation of TTBK2’s role in TDP-43 phosphorylation.
Snippet: S4 Figure. In vitro kinase assay controls. (A) Tau is a known substrate of TTBK2 [65]. To test enzyme activity, approximately 1 µg of non-phosphorylated recombinant human tau purified from E. coli were incubated with equivalent amounts of TTBK2 enzyme purified from cultured cells by two commercial suppliers (Origene catalog #LY406582 and Signalchem #T18-11G)
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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