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Cyclin D3 (CCND3) (NM_001760) Human Over-expression Lysate

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SKU
LY400666
Transient overexpression lysate of cyclin D3 (CCND3), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202437
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001760, NP_001751
Predicted MW 32.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001751
Locus ID 896
Cytogenetics 6p21.1
Protein Families WB
Protein Pathways Cell cycle, Focal adhesion, Jak-STAT signaling pathway, p53 signaling pathway, Wnt signaling pathway
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Other "Cyclin D3" proteins (2)
SKU Description Size Price
LC400666 Lyophilized CCND3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP761887 Purified recombinant protein of Human cyclin D3 (CCND3), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

L-arginine availability regulates T-lymphocyte cell-cycle progression
Rodriguez, Quiceno, Ochoa
Blood (2007) 109 (4), 1568-73 DOI: 10.1182/blood-2006-06-031856
Cited by 922
AI Summary: In this study, OriGene’s cyclin D3 siRNA (catalog # SR304436) was used to specifically silence cyclin D3 expression in T cells, demonstrating that reduced cyclin D3 alone could reproduce the cell cycle arrest observed during L-arginine starvation.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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