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KK LC 1 (CT83) (NM_001017978) Human Over-expression Lysate

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SKU
LY400409
Transient overexpression lysate of chromosome X open reading frame 61 (CXorf61) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209391
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001017978, NP_001017978
Synonyms CXorf61; KK-LC-1; KKLC1
Predicted MW 12.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001017978
Locus ID 203413
Cytogenetics Xq23
Protein Families WB
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Other "KK LC 1" proteins (3)
SKU Description Size Price
PH309391 CXorf61 MS Standard C13 and N15-labeled recombinant protein (NP_001017978) 10 ug
$3,360.00
LC400409 Lyophilized CT83 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309391 Recombinant protein of human chromosome X open reading frame 61 (CXorf61), 20 µg 20 ug
$867.00

file-alt Citations

Critical role of spns2, a sphingosine-1-phosphate transporter, in lung cancer cell survival and migration
Bradley, Dasgupta, Jiang et al
PLoS ONE (2014) 9 (10), e110119 DOI: 10.1371/journal.pone.0110119
Cited by 63
AI Summary: OriGene’s Human Lung Cancer real-time PCR arrays were used in this study to quantify Spns2 mRNA levels in lung cancer patient samples, helping to demonstrate that Spns2 expression is reduced in advanced lung cancer.
Snippet: The PI3K inhibitor LY294002 was from Cayman (Ann Arbor, MI). And the Jak inhibitor JAKi was from Millipore (Billerica, MA). Human LC real time PCR arrays were from Origene (Rockville, MD). Methods. Plasmid cloning. Human ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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