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Acid sphingomyelinase (SMPD1) (NM_000543) Human Over-expression Lysate

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SKU
LY400191
Transient overexpression lysate of sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant ASM-1 (as WB positive control)
  $665.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219758
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000543, NP_000534
Synonyms ASM; ASMASE; NPD
Predicted MW 69.94 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000534
Locus ID 6609
Cytogenetics 11p15.4
Protein Families WB
Protein Pathways Lysosome, Metabolic pathways, Sphingolipid metabolism
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Other "Acid sphingomyelinase" proteins (5)
SKU Description Size Price
PH319758 SMPD1 MS Standard C13 and N15-labeled recombinant protein (NP_000534) 10 ug
$3,360.00
LC400191 Lyophilized SMPD1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423515 Lyophilized SMPD1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY423515 Transient overexpression lysate of sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant ASM-2 (as WB positive control) 100 ug
$665.00
TP319758 Purified recombinant protein of Homo sapiens sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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