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Aromatase (CYP19A1) (NM_000103) Human Over-expression Lysate

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SKU
LY400031
Transient overexpression lysate of cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1 (as WB positive control)
  $436.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205890
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000103, NP_000094
Synonyms ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
Predicted MW 57.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000094
Locus ID 1588
Cytogenetics 15q21.2
Protein Families WB
Protein Pathways Androgen and estrogen metabolism, Metabolic pathways
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Other "Aromatase" proteins (5)
SKU Description Size Price
LC400031 Lyophilized CYP19A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC410595 Lyophilized CYP19A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY410595 Transient overexpression lysate of cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP305890 Purified recombinant protein of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1, full length, with C-terminal MYC/DDK tag, expressed in HEK293 cells, 20 µg 20 ug
$737.00
TP762150 Purified recombinant protein of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1,Tyr241-end, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Prognostic relevance of estrogen receptor α, β and aromatase expression in non-small cell lung cancer
Skjefstad, Grindstad, Khanehkenari et al
Steroids (2016)
Cited by 53
AI Summary: OriGene products—including HEK 293 over-expressed cell lysates for ERα (LY400046), ERβ1 (LY425704), and CYP-19 (LY400031)—were used as controls in immunohistochemical analyses to evaluate hormone receptor expression in non-small cell lung cancer tissues.
Snippet: For further evaluation, HEK 293 over-expressed cell lysates were utilized also from OriGene for ERα (LY400046), ERβ1(LY425704) and CYP-19(LY400031) and were incubated with 2xSDS Sample Buffer for 10 minutes at 100°C. Equal amounts of protein lysates were ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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