Plaur (BC010309) Mouse Over-expression Lysate

SKU
LC702555
Plaur HEK293T cell transient overexpression lysate (as WB positive control)
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone MR202555
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN BC010309
Synonyms u-PAR, uPAR, Cd87
Predicted MW 24.7 kDa
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
Locus ID 18793
Cytogenetics 7 A3
Protein Families WB
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Other "Plaur" proteins (2)
SKU Description Size Price
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TP502555 Purified recombinant protein of Mouse plasminogen activator, urokinase receptor (cDNA clone MGC:11631 IMAGE:3158012), full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug 20 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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